Biotechnology - Principles (Genetic Engineering, Restriction Enzymes, Cloning Vectors)

Grade 12 Biology — Biotechnology - Principles (Genetic Engineering, Restriction Enzymes, Cloning Vectors): 25 practice questions with instant scoring and explanations.

1. What is the primary function of restriction enzymes in genetic engineering?
2. Which of the following is NOT a cloning vector?
3. What are sticky ends in DNA recombination?
4. Which enzyme is responsible for joining DNA fragments together?
5. What is the minimum size of DNA insert that a plasmid can typically carry?
6. Which restriction enzyme recognizes and cuts palindromic sequences?
7. What is a recombinant DNA molecule?
8. Which of the following is an advantage of using plasmids as vectors?
9. What is the recognition sequence for the restriction enzyme EcoRI?
10. How do blunt ends differ from sticky ends?
11. What is the main advantage of using bacteriophages as vectors?
12. Which of the following statements about cosmids is true?
13. What is the purpose of a selectable marker gene in a cloning vector?
14. Which of the following is an example of a selectable marker?
15. What is the role of DNA polymerase in genetic engineering?
16. What is a multiple cloning site (MCS) in a vector?
17. Which enzyme can produce blunt ends from sticky ends?
18. What is the significance of the origin of replication in a cloning vector?
19. Which of the following best describes genetic engineering?
20. What is the purpose of using isocaudidate ends in DNA cloning?
21. Which of the following is NOT a characteristic of a good cloning vector?
22. What is the difference between in vitro and in vivo genetic manipulation?
23. How does a restriction enzyme recognize its target sequence?
24. What is the typical range of DNA insert size that plasmids can accommodate?
25. Which of the following describes the function of topoisomerase in genetic engineering?